GlenResearch 10-1001說明書

發布時間：2018/8/15

點擊次數：496

美國GRC公司：Glen Research Corp 創建于1987年，專注于為DNA和RNA寡核苷酸的化學合成，修飾，標記和純化提供zui廣泛的亞磷酰胺和固體支持物，現已有近三十年的歷史，在低聚核苷酸及修飾、改性領域一直處于地位。

結構研究

以下產品用于研究當關鍵結構元件改變時對寡核苷酸活性的影響。7-脫氮嘌呤單體缺乏對氫鍵合至關重要的基團。7-Deaza-8-aza-A和7-deaza-8-aza-G（PPG）單體是A和G的異構體，并且具有相似的電子密度。它們在寡核苷酸中的存在相對于A和G略微穩定。與G不同，PPG不會導致聚集，并且可以容易地制備和分離富含G的寡核苷酸。在5'-末端具有PPG的5'-熒光素寡核苷酸比等同的G寡核苷酸淬滅得少得多。作為胸苷的嘌呤類似物，7-脫氮-2'-脫氧黃嘌呤核苷（7-deaza-dX）有望對三鏈體的DNA結構產生有趣的影響。7-Deaza-dX還與2,4-二氨基嘧啶核苷類似物形成非標準堿基對。標準核堿基具有未共享的電子對，其突出到雙鏈DNA的小溝中。與DNA，聚合酶，逆轉錄酶，限制酶等相互作用的酶可以使用氫鍵供體基團與小溝中的氫鍵受體接觸。3-Deaza-2'-deoxyadenosine非常有趣，因為它保持了常規Watson-Crick氫與T鍵合的能力，但缺乏通常由N3提供的3-位的電子對。

GlenResearch 目錄號：10-1001-xx

7-Deaza-dA-CE Phosphoramidite
描述：7- Deaza -dA-CE亞磷酰胺

5'-二甲氧基三苯甲基-N-苯甲?；?7-脫氮-2'-脫氧腺苷，3' - [（2-氰基乙基） - （N，N-二異丙基）] - 亞磷酰胺

分子式：C _4 8 H _5 3 N ₆ O ₇ P.

MW：856.96

FW：312.22

稀釋劑：無水乙腈

耦合：合成器制造商推薦的標準方法無需更改。

去保護：合成器制造商推薦的標準方法無需更改。

儲存：冷藏，高2-8°C，干燥

溶液中的穩定性：類似于dA，C，G，T-CE亞磷酰胺

10-1001-95

50µm

10-1001-90

100µm

10-1001-02

0.25g

EXTINCTION DATA

Item	Nucleoside	λMax-1	Emax-1	λMax-2	Emax-2	E260
		(nm)	(ml/µmole)	nm	(ml/µmole)	(ml/µmole)
10-1001	7-deaza-dA	270	11.3			9.4

DILUTION/COUPLING DATA

下表顯示了包裝尺寸數據，以及基于正常灌注程序的溶液，稀釋液和近似偶聯。請與各種合成器鏈接以獲取更詳細的使用信息。

ABI 392/394
Cat.No.	Pack Size	Grams/ Pack	0.1M Dil. (mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
Cat.No.	Pack Size	Grams/ Pack	0.1M Dil. (mL)	Approximate Number of Additions
10-1001-02	0.25grams	.25grams	2.92	84	50.4	31.5	22.91	16.8	4.2
10-1001-90	100µmoles	.086grams	1	20	12	7.5	5.45	4	1
10-1001-95	50µmoles	.043grams	.5	3.33	2	1.25	.91	.67	.17
Expedite
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Molarity	50nm	0.2µm	1µm	15µm
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Approximate Number of Additions
10-1001-02	0.25grams	.25grams	4.35	.07	80.6	50.38	36.64	5.04
10-1001-90	100µmoles	.086grams	1.5	.07	23.6	14.75	10.73	1.48
10-1001-95	50µmoles	.043grams	.75	.07	8.6	5.38	3.91	.54

貨號	品名	規格	目錄價	品牌
10-0001-02	dA-5'-CE Phosphoramidite	0.25grams	1275	glen research
10-0001-05	dA-5'-CE Phosphoramidite	0.5grams	2550	glen research
10-0001-10	dA-5'-CE Phosphoramidite	1.0gram	5100	glen research
10-0101-02	dC-5'-CE Phosphoramidite	0.25grams	1275	glen research
10-0101-05	dC-5'-CE Phosphoramidite	0.5grams	2550	glen research
10-0101-10	dC-5'-CE Phosphoramidite	1.0gram	5100	glen research
10-0301-02	dT-5'-CE Phosphoramidite	0.25grams	1275	glen research
10-0301-05	dT-5'-CE Phosphoramidite	0.5grams	2550	glen research
10-0301-10	dT-5'-CE Phosphoramidite	1.0gram	5100	glen research
10-1000-02	dA-CE Phosphoramidite	0.25grams	212.5	glen research
10-1000-02M	dA-CE Phosphoramidite	0.25grams	212.5	glen research
10-1000-05	dA-CE Phosphoramidite	0.5grams	425	glen research
10-1000-05M	dA-CE Phosphoramidite	0.5grams	425	glen research
10-1000-10	dA-CE Phosphoramidite	1.0gram	850	glen research
10-1000-10M	dA-CE Phosphoramidite	1.0gram	850	glen research
10-1000-1C	dA-CE Phosphoramidite	1.0gram	850	glen research
10-1000-1S	dA-CE Phosphoramidite	10.0grams	8500	glen research
10-1000-20	dA-CE Phosphoramidite	2.0grams	1700	glen research
10-1000-2C	dA-CE Phosphoramidite	2.0grams	1700	glen research
10-1000-40	dA-CE Phosphoramidite	4.0grams	3400	glen research
10-1000-50	dA-CE Phosphoramidite	5.0grams	4250	glen research
10-1000-5S	dA-CE Phosphoramidite	5.0grams	4250	glen research
10-1000-C2	dA-CE Phosphoramidite	0.25grams	212.5	glen research
10-1000-C5	dA-CE Phosphoramidite	0.5grams	425	glen research
10-1001-02	7-Deaza-dA-CE Phosphoramidite	0.25grams	16575	glen research
10-1001-90	7-Deaza-dA-CE Phosphoramidite	100µmoles	6035	glen research
10-1001-95	7-Deaza-dA-CE Phosphoramidite	50µmoles	3017.5	glen research
10-1002-02	dA+dG-CE Phosphoramidite	0.25grams	680	glen research
10-1003-02	N6-Me-dA-CE Phosphoramidite	0.25grams	8415	glen research
10-1003-90	N6-Me-dA-CE Phosphoramidite	100µmoles	2762.5	glen research
10-1004-02	3'-dA-CE Phosphoramidite	0.25grams	16575	glen research
10-1004-90	3'-dA-CE Phosphoramidite	100µmoles	6035	glen research
10-1004-95	3'-dA-CE Phosphoramidite	50µmoles	3017.5	glen research
10-1006-02	Etheno-dA-CE Phosphoramidite	0.25grams	4335	glen research
10-1006-90	Etheno-dA-CE Phosphoramidite	100µmoles	1785	glen research
10-1007-02	8-Br-dA-CE Phosphoramidite	0.25grams	5015	glen research
10-1007-90	8-Br-dA-CE Phosphoramidite	100µmoles	1955	glen research
10-1008-02	8-oxo-dA-CE Phosphoramidite	0.25grams	6035	glen research
10-1008-90	8-oxo-dA-CE Phosphoramidite	100µmoles	2295	glen research
10-1010-02	dC-CE Phosphoramidite	0.25grams	212.5	glen research
10-1010-02M	dC-CE Phosphoramidite	0.25grams	212.5	glen research
10-1010-05	dC-CE Phosphoramidite	0.5grams	425	glen research
10-1010-05M	dC-CE Phosphoramidite	0.5grams	425	glen research
10-1010-10	dC-CE Phosphoramidite	1.0gram	850	glen research
10-1010-10M	dC-CE Phosphoramidite	1.0gram	850	glen research
10-1010-1C	dC-CE Phosphoramidite	1.0gram	850	glen research
10-1010-1S	dC-CE Phosphoramidite	10.0grams	8500	glen research
10-1010-20	dC-CE Phosphoramidite	2.0grams	1700	glen research
10-1010-2C	dC-CE Phosphoramidite	2.0grams	1700	glen research
10-1010-40	dC-CE Phosphoramidite	4.0grams	3400	glen research
10-1010-50	dC-CE Phosphoramidite	5.0grams	4250	glen research
10-1010-5S	dC-CE Phosphoramidite	5.0grams	4250	glen research
10-1010-C2	dC-CE Phosphoramidite	0.25grams	212.5	glen research
10-1010-C5	dC-CE Phosphoramidite	0.5grams	425	glen research
10-1013-02	dC+dT-CE Phosphoramidite	0.25grams	680	glen research
10-1014-02	pdC-CE Phosphoramidite	0.25grams	4165	glen research
10-1014-05	pdC-CE Phosphoramidite	0.5grams	8330	glen research
10-1014-90	pdC-CE Phosphoramidite	100µmoles	1445	glen research
10-1015-02	Ac-dC-CE Phosphoramidite	0.25grams	212.5	glen research
10-1015-02M	Ac-dC-CE Phosphoramidite	0.25grams	212.5	glen research
10-1015-05	Ac-dC-CE Phosphoramidite	0.5grams	425	glen research
10-1015-05M	Ac-dC-CE Phosphoramidite	0.5grams	425	glen research
10-1015-10	Ac-dC-CE Phosphoramidite	1.0gram	850	glen research
10-1015-10M	Ac-dC-CE Phosphoramidite	1.0gram	850	glen research
10-1015-1C	Ac-dC-CE Phosphoramidite	1.0gram	850	glen research
10-1015-1S	Ac-dC-CE Phosphoramidite	10.0grams	8500	glen research
10-1015-20	Ac-dC-CE Phosphoramidite	2.0grams	1700	glen research
10-1015-2C	Ac-dC-CE Phosphoramidite	2.0grams	1700	glen research
10-1015-40	Ac-dC-CE Phosphoramidite	4.0grams	3400	glen research
10-1015-50	Ac-dC-CE Phosphoramidite	5.0grams	4250	glen research
10-1015-5S	Ac-dC-CE Phosphoramidite	5.0grams	4250	glen research
10-1015-C2	Ac-dC-CE Phosphoramidite	0.25grams	212.5	glen research
10-1015-C5	Ac-dC-CE Phosphoramidite	0.5grams	425	glen research
10-1016-02	TMP-F-dU-CE Phosphoramidite	0.25grams	8415	glen research
10-1016-90	TMP-F-dU-CE Phosphoramidite	100µmoles	3315	glen research
10-1017-02	Pyrrolo-dC-CE Phosphoramidite	0.25grams	11475	glen research
10-1017-90	Pyrrolo-dC-CE Phosphoramidite	100µmoles	3740	glen research
10-1017-95	Pyrrolo-dC-CE Phosphoramidite	50µmoles	1870	glen research
10-1018-02	5-Me-dC Brancher Phosphoramidite	0.25grams	8585	glen research
10-1018-90	5-Me-dC Brancher Phosphoramidite	100µmoles	3485	glen research
10-1019-02	Amino-Modifier C6 dC	0.25grams	7650	glen research
10-1019-90	Amino-Modifier C6 dC	100µmoles	3825	glen research
10-1020-02	dG-CE Phosphoramidite	0.25grams	212.5	glen research
10-1020-02M	dG-CE Phosphoramidite	0.25grams	212.5	glen research
10-1020-05	dG-CE Phosphoramidite	0.5grams	425	glen research
10-1020-05M	dG-CE Phosphoramidite	0.5grams	425	glen research
10-1020-10	dG-CE Phosphoramidite	1.0gram	850	glen research
10-1020-10M	dG-CE Phosphoramidite	1.0gram	850	glen research
10-1020-1C	dG-CE Phosphoramidite	1.0gram	850	glen research
10-1020-1S	dG-CE Phosphoramidite	10.0grams	8500	glen research
10-1020-20	dG-CE Phosphoramidite	2.0grams	1700	glen research
10-1020-2C	dG-CE Phosphoramidite	2.0grams	1700	glen research
10-1020-40	dG-CE Phosphoramidite	4.0grams	3400	glen research
10-1020-50	dG-CE Phosphoramidite	5.0grams	4250	glen research
10-1020-5S	dG-CE Phosphoramidite	5.0grams	4250	glen research
10-1020-C2	dG-CE Phosphoramidite	0.25grams	212.5	glen research
10-1020-C5	dG-CE Phosphoramidite	0.5grams	425	glen research
10-1021-02	7-deaza-dG-CE Phosphoramidite	0.25grams	16575	glen research
10-1021-90	7-deaza-dG-CE Phosphoramidite	100µmoles	6035	glen research
10-1021-95	7-deaza-dG-CE Phosphoramidite	50µmoles	3017.5	glen research
10-1023	dA+dC+dG+dT-CE Phosphoramidite Mix	1.0gram	680	glen research
10-1027-02	8-Br-dG-CE Phosphoramidite	0.25grams	4335	glen research
10-1027-90	8-Br-dG-CE Phosphoramidite	100µmoles	1785	glen research
10-1028-02	8-oxo-dG-CE Phosphoramidite	0.25grams	16575	glen research
10-1028-90	8-oxo-dG-CE Phosphoramidite	100µmoles	6035	glen research
10-1028-95	8-oxo-dG-CE Phosphoramidite	50µmoles	3017.5	glen research
10-1029-02	dmf-dG-CE Phosphoramidite	0.25grams	212.5	glen research
10-1029-02M	dmf-dG-CE Phosphoramidite	0.25grams	212.5	glen research
10-1029-05	dmf-dG-CE Phosphoramidite	0.5grams	425	glen research
10-1029-05M	dmf-dG-CE Phosphoramidite	0.5grams	425	glen research
10-1029-10	dmf-dG-CE Phosphoramidite	1.0gram	850	glen research
10-1029-10M	dmf-dG-CE Phosphoramidite	1.0gram	850	glen research
10-1029-1C	dmf-dG-CE Phosphoramidite	1.0gram	850	glen research
10-1029-1S	dmf-dG-CE Phosphoramidite	10.0grams	8500	glen research
10-1029-20	dmf-dG-CE Phosphoramidite	2.0grams	1700	glen research
10-1029-2C	dmf-dG-CE Phosphoramidite	2.0grams	1700	glen research
10-1029-40	dmf-dG-CE Phosphoramidite	4.0grams	3400	glen research
10-1029-50	dmf-dG-CE Phosphoramidite	5.0grams	4250	glen research
10-1029-5S	dmf-dG-CE Phosphoramidite	5.0grams	4250	glen research
10-1029-C2	dmf-dG-CE Phosphoramidite	0.25grams	212.5	glen research
10-1029-C5	dmf-dG-CE Phosphoramidite	0.5grams	425	glen research
10-1030-02	dT-CE Phosphoramidite	0.25grams	212.5	glen research
10-1030-02M	dT-CE Phosphoramidite	0.25grams	212.5	glen research
10-1030-05	dT-CE Phosphoramidite	0.5grams	425	glen research
10-1030-05M	dT-CE Phosphoramidite	0.5grams	425	glen research
10-1030-10	dT-CE Phosphoramidite	1.0gram	850	glen research
10-1030-10M	dT-CE Phosphoramidite	1.0gram	850	glen research
10-1030-1C	dT-CE Phosphoramidite	1.0gram	850	glen research
10-1030-1S	dT-CE Phosphoramidite	10.0grams	8500	glen research
10-1030-20	dT-CE Phosphoramidite	2.0grams	1700	glen research
10-1030-2C	dT-CE Phosphoramidite	2.0grams	1700	glen research
10-1030-40	dT-CE Phosphoramidite	4.0grams	3400	glen research
10-1030-50	dT-CE Phosphoramidite	5.0grams	4250	glen research
10-1030-5S	dT-CE Phosphoramidite	5.0grams	4250	glen research
10-1030-C2	dT-CE Phosphoramidite	0.25grams	212.5	glen research
10-1030-C5	dT-CE Phosphoramidite	0.5grams	425	glen research
10-1031-02	5'-OMe-dT-CE Phosphoramidite	0.25grams	6035	glen research
10-1031-90	5'-OMe-dT-CE Phosphoramidite	100µmoles	2295	glen research
10-1032-02	O4-Me-dT-CE Phosphoramidite	0.25grams	6035	glen research
10-1032-90	O4-Me-dT-CE Phosphoramidite	100µmoles	2295	glen research
10-1034-02	4-Thio-dT-CE Phosphoramidite	0.25grams	11475	glen research
10-1034-90	4-Thio-dT-CE Phosphoramidite	100µmoles	5015	glen research
10-1034-95	4-Thio-dT-CE Phosphoramidite	50µmoles	2805	glen research
10-1035-02	Carboxy-dT	0.25grams	6120	glen research
10-1035-90	Carboxy-dT	100µmoles	3060	glen research
10-1036-02	2-Thio-dT-CE Phosphoramidite	0.25grams	11475	glen research
10-1036-90	2-Thio-dT-CE Phosphoramidite	100µmoles	5015	glen research
10-1036-95	2-Thio-dT-CE Phosphoramidite	50µmoles	2805	glen research
10-1037-02	Amino-Modifier C2 dT	0.25grams	6120	glen research
10-1037-05	Amino-Modifier C2 dT	0.5grams	12240	glen research
10-1037-90	Amino-Modifier C2 dT	100µmoles	3060	glen research
10-1038-02	Biotin-dT	0.25grams	10625	glen research
10-1038-90	Biotin-dT	100µmoles	5525	glen research
10-1038-95	Biotin-dT	50µmoles	2847.5	glen research
10-1039-02	Amino-Modifier C6 dT	0.25grams	6120	glen research
10-1039-05	Amino-Modifier C6 dT	0.5grams	12240	glen research
10-1039-90	Amino-Modifier C6 dT	100µmoles	3060	glen research
10-1040-02	dI-CE Phosphoramidite	0.25grams	2040	glen research
10-1040-90	dI-CE Phosphoramidite	100µmoles	850	glen research
10-1041-02	2'-DeoxyNebularine-CE Phosphoramidite (Purine)	0.25grams	4335	glen research
10-1041-90	2'-DeoxyNebularine-CE Phosphoramidite (Purine)	100µmoles	1785	glen research
10-1042-02	O6-Phenyl-dI-CE Phosphoramidite	0.25grams	6035	glen research
10-1042-90	O6-Phenyl-dI-CE Phosphoramidite	100µmoles	2295	glen research
10-1044-02	5-Nitroindole-CE Phosphoramidite	0.25grams	5525	glen research
10-1044-90	5-Nitroindole-CE Phosphoramidite	100µmoles	2125	glen research
10-1046-02	2-Aminopurine-CE Phosphoramidite	0.25grams	6035	glen research
10-1046-90	2-Aminopurine-CE Phosphoramidite	100µmoles	2295	glen research
10-1047-02	dP-CE Phosphoramidite	0.25grams	10115	glen research
10-1047-90	dP-CE Phosphoramidite	100µmoles	3315	glen research
10-1048-02	dK-CE Phosphoramidite	0.25grams	10115	glen research
10-1048-90	dK-CE Phosphoramidite	100µmoles	3315	glen research
10-1049-02	dP+dK-CE Phosphoramidite	0.25grams	10115	glen research
10-1049-90	dP+dK-CE Phosphoramidite	100µmoles	3315	glen research
10-1050-02	dU-CE Phosphoramidite	0.25grams	1700	glen research
10-1050-90	dU-CE Phosphoramidite	100µmoles	595	glen research
10-1051-02	O4-Triazolyl-dU-CE Phosphoramidite	0.25grams	6035	glen research
10-1051-90	O4-Triazolyl-dU-CE Phosphoramidite	100µmoles	2295	glen research
10-1052-02	4-Thio-dU-CE Phosphoramidite	0.25grams	11475	glen research
10-1052-90	4-Thio-dU-CE Phosphoramidite	100µmoles	5015	glen research
10-1052-95	4-Thio-dU-CE Phosphoramidite	50µmoles	2805	glen research
10-1053-02	5-OH-dU-CE Phosphoramidite	0.25grams	11475	glen research
10-1053-90	5-OH-dU-CE Phosphoramidite	100µmoles	3825	glen research
10-1054-02	pdU-CE Phosphoramidite	0.25grams	3315	glen research
10-1054-05	pdU-CE Phosphoramidite	0.5grams	6630	glen research
10-1054-90	pdU-CE Phosphoramidite	100µmoles	1105	glen research
10-1055-02	2'-deoxypseudoU-CE Phosphoramidite	0.25grams	16575	glen research
10-1055-90	2'-deoxypseudoU-CE Phosphoramidite	100µmoles	6035	glen research
10-1055-95	2'-deoxypseudoU-CE Phosphoramidite	50µmoles	3017.5	glen research
10-1056-02	Fluorescein-dT Phosphoramidite	0.25grams	11475	glen research

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GlenResearch 10-1001說明書

美國GRC公司：Glen Research Corp 創建于1987年，專注于為DNA和RNA寡核苷酸的化學合成，修飾，標記和純化提供zui廣泛的亞磷酰胺和固體支持物，現已有近三十年的歷史，在低聚核苷酸及修飾、改性領域一直處于地位。

結構研究

GlenResearch 目錄號：10-1001-xx

7-Deaza-dA-CE Phosphoramidite描述：7- Deaza -dA-CE亞磷酰胺

EXTINCTION DATA

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

美國GRC公司：Glen Research Corp 創建于1987年，專注于為DNA和RNA寡核苷酸的化學合成，修飾，標記和純化提供zui廣泛的亞磷酰胺和固體支持物，現已有近三十年的歷史，在低聚核苷酸及修飾、改性領域一直處于地位。

7-Deaza-dA-CE Phosphoramidite
描述：7- Deaza -dA-CE亞磷酰胺