5-甲基胞苷-5-三磷酸鹽是TRILINK公司產(chǎn)品，用于干細胞研究用

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5-甲基胞苷-5-三磷酸鹽是TRILINK公司新產(chǎn)品，用于干細胞研究用,5-methyl-ctp

Synonyms]
5-methyl-dCTP
5-methyldeoxycytidine triphosphate
CPD-1094
5-methyl deoxycytidine-5'-triphosphate
5-methyl-2'-deoxycytidine-5'-triphosphate
cytidine 5'-（tetrahydrogen triphosphate）, 2'-deoxy-5-methyl-
[[[5-[（4-amino-5-methyl-2-oxo-1H-pyrimidin-1-yl）]-3-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxyphosphonic acid

[Structure]

[ Properties Computed from Structure]

Molecular Weight	481.183503 [g/mol]
Molecular Formula	C₁₀H₁₈N₃O₁₃P₃
XLogP	-5.9
H-Bond Donor	6
H-Bond Acceptor	14
Rotatable Bond Count	8
Tautomer Count	3
Exact Mass	481.005247
MonoIsotopic Mass	481.005247
Topological Polar Surface Area	248
Heavy Atom Count	29
Formal Charge	0
Complexity	868
Isotope Atom Count	0
Defined Atom StereoCenter Count	0
Undefined Atom StereoCenter Count	3
Defined Bond StereoCenter Count	0
Undefined Bond StereoCenter Count	0
Covalently-Bonded Unit Count	1

Description
5-Methyl-dCTP is widely used for construction of cDNA libraries

Incorporation of 5-Methyl-dCTP
M-MuLV Reverse Transcriptase
Klenow Fragment of DNA Polymerase I
Sequenase DNA Polymerase
（Taq Polymerase, Vent） *
Incorporation of Hg-dCTP
DNA Polymerase I

References to 5-Methyl-dCTP
Lefaucheur et al. （1998） Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584.
Nelson et al. （1993） Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 （3）:681.
Asamizu et al. （1999） A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research6:369.
* Wong et al. （1991） PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 （5）:1081.
Reference to Hg-dCTP
Banfalvi et al. （1995） Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 （5）:445.

N-1014-1	5-Methylcytidine-5'-TP	1umole	1317.5
N-1014-10	5-Methylcytidine-5'-TP	10umoles	10625
N-1014-5	5-Methylcytidine-5'-TP	5umoles	6205

Reference（s）
Kariko K, Muramatsu H, Ludwig J, Weissman D. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. （2011） Nucleic Acids Research.

Kormann M, Hasenpusch G, Aneja M, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. （2011） Nature Biotechnology 29:154–157.

Anderson, B., Muramatsu, H., Nallagatla, S.R., Bevilacqua, P.C., Sansing, L.H., Weissman, D. & Kariko, K. Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation （2010） Nucleic Acids Research, 38（17）: 5884-5892.

Warren et al., Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell （2010）, doi:10.1016/j.stem.2010.08.012.

Kariko K, Muramatsu H, Welsh F, et al. Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. （2008） Molecular Therapy （16）11: 1833-1840.

Kariko, K., Buckstein, M., Ni, H. & Weissman, D. Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA （2005）. Immunity, 23（2）, 165-175.

Lefmann M, et al. Novel Mass Spectrometry-based tool for genotypic identification of mycobacteria. （2004） Journal of Clinical Microbiology, 42（1）: 339-346.

Hartmer R, Storm N, Boecker S, Rodi CP, Hillenkamp F, Jurinke C, van den Boom D. RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. （2003） Nucleic Acids Res., 31（9）: e47.

Nguyen A, Zhao C, Dorris D, Mazumder A. Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. （2002） BMC Biotechnology, 2（1）: 14.

Van Rompay AR, Norda A, Linden K, Johansson M, Karlsson A. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. （2001） Mol Pharmacol., 59（5）: 1181-6.